Journal: Nature structural & molecular biology

Article Title: DELE1 oligomerization promotes integrated stress response activation

doi: 10.1038/s41594-023-01061-0

Figure Lengend Snippet: Cryo-EM data collection, refinement, and validation

Article Snippet: Table 1 | EMD-27269 PDB 8D9X Data collection Microscope Talos Arctica Voltage (keV) 200 Detector K2 Summit (Counting) Magnification (nominal/calibrated) ×36,000/×43,478 Exposure navigation Image shift to 16 holes Data acquisition software Leginon Total electron exposure (e − /Å 2 ) 50 Exposure rate (e − /pixel/sec) 6.75 Frame length (ms) 200 Number of frames per micrograph 49 Pixel size (Å) 1.15 Defocus range (μm) −0.7 to −1.2 Micrographs collected (no.) 13,164 Reconstruction Image processing package cryoSPARC Total extracted particles (no.) 1,060,000 Refined particles (no.) 92,455 Final particles (no.) 67,067 Symmetry imposed D 4 Resolution (Å) FSC 0.5 (unmasked masked) 4.5/4.0 FSC 0.143 (unmasked/masked) 4.03/3.8 Resolution range (local) 3.3–4.5 3DFSC sphericity 0.9 of 1 Sharpening B-factor (Å 2 ) −171.6 Model composition Protein residues 1,680 Ligands 0 Model refinement Refinement package Phenix CC (volume/mask) 0.71/0.71 R.m.s. deviations Bond lengths 0.003 Bond angles (°) 0.678 Validation Map-to-model FSC 0.5 4.1 Ramachandran (%) Outliers 0 Allowed 3.19 Favored 96.81 MolProbity score 1.79 Poor rotamers (%) 0.00 Clashscore (all atoms) 11.98 C-beta deviations 0.00 CaBLAM Outliers (%) 22 0.49 EMRinger score 1.56 Open in a separate window Cryo-EM data collection, refinement, and validation.

Techniques: Microscopy, Software